Category: Mass Spectrometry Based Proteomics Analysis Certification Course

The main significance of proteomics research is studying the phenomenon of life and promoting human health. Protein is the executor of physiological function and the direct embodiment of life. The study of protein structure and function will directly clarify the mechanism of life under physiological or pathological conditions. Some phenomena of life are not only determined by a certain protein, but also by the interaction of multiple proteins, which requires the study of proteomics.

Performing mass spectrometry based proteomics requires background knowledge and experience. Also, understanding and handling mass spectrometry data is a multifaceted task that requires many decisions to be made to get the most comprehensive information from an experiment.

In this course, we therefore aim to provide an overview of state-of-art techniques for the analysis and characterization of protein and peptide samples by mass spectrometry (MS/MS). 

This course will surely help in understanding concepts like:

• in-depth basics of mass spectrometry
• sample preparation for analysis in mass spectrometer
• type of instrumentations,
• fragmentation method
• quantitative proteomics techniques such as iTRAQ, SILAC and TMT using mass spectrometry
• analysis strategy best suited to an individual sample as this technique is mostly used in proteomics based research nowadays.

Also, how mass spectrometer can be used to accurately distinguish different isoforms or modified forms of proteins (Post translational modification), even in mixtures, that can provide added dimensions in the design of quantitative strategies and simplifies multiplex assay development.

Before proceeding with Hands-on training for any such technique a thorough understanding of the basics & its working principle is very much important.

With the Knowledge of Basic Concepts + Working Principle + Hands-On Experience in Mass Spectrometry mentioned in your CV, that dream research job of yours will not be far away.

Eligibility For This Course:

• Students in their B.Sc / B.Tech / M.Sc / M.Tech who aspire to work on cutting-edge research technologies.
• Aspiring scientists who look forward to innovating Proteomics approaches.
• Science enthusiasts who like to stay updated about the recent research updates using the most talked about processes.
• Any candidate who is looking for a career in the pharmaceutical & healthcare industry

BioTecNika brings to you Certification Course on “Mass Spectrometry-Based Proteomics Analysis” on special demand by our subscribers.

This Certification program is co-sponsored by PEAK Instruments Inc ,a leading manufacturer of Analytical instruments.

Proteins are essential parts of living organisms, with many functions.  The proteome is a term that is used for the entire set of proteins that is or can be expressed by a genome, cell, tissue, or organism at a certain time. The large-scale study of proteins is known as Proteomics. The proteome is not only complex, it is spatially, temporally, and chemically dynamic.

Mass spectrometry is an advanced analytical technique for accurate mass measurement. It does so by producing charged molecular species in vacuum followed by its separation in the magnetic and electric fields on the basis of their mass to charge (m/z) ratio.

Mass spectrometry (MS)-based proteomics is emerging as a broadly effective means for identification, characterization, and quantification of proteins that are integral components of the processes essential for life. Characterization of proteins at the proteome and sub-proteome (e.g., the phosphoproteome, proteoglycome, or degradome/peptidome) levels provides a foundation for understanding fundamental aspects of biology. Among the toolkit of techniques with which proteins can be investigated on a large scale, mass spectrometry (MS) has gained popularity because of its ability to handle the complexities associated with the proteome. Other techniques such as two-dimensional gel electrophoresis (2DE), two-hybrid analysis, and protein microarrays fail to achieve the depth of informative proteome analysis seen with mass spectrometry.

The three primary applications of MS to proteomics are :

• cataloging protein expression
• defining protein interactions
• and identifying sites of protein modification.

The use of MS for proteomics is not the application of a single technique for all purposes but rather a collection of methodologies, each with strengths suited to particular inquiries. MS-based proteomics is increasingly contribute to our understanding of the dynamics, interactions, and roles that proteins and peptides play, advancing our understanding of biology on a systems wide level for a wide range of applications including investigations of microbial communities, bioremediation, and human health.

Fig: General workflow for MS-based high-throughput bottom-up and top-down proteomics.

Common applications and fields that use mass spectrometry:

Proteomics

• Protein structure, function & folding determination
• Identify a protein from the mass of its peptide fragments.
• Detection of specific post-translational modifications in complex biological mixtures.
• Quantitate proteins in a given sample.
• Monitor enzyme reactions, chemical modifications & protein digestion.

Drug Discovery

• Determination & Screening of structures of drugs & metabolites.

Clinical Testing

• In Forensic Analysis
• Detect disease bio-markers (e.g., newborns screened for metabolic diseases).

Genomics

• Sequencing short oligonucleotides.

Environment

• Test food &beverages for contamination, adulteration and

About Peak Instruments Inc.:

PEAK Instruments Inc. is Houston with its head office and warehouse, which is a high tech enterprise integrated with R&D, production, sales and service of spectrophotometers.  Spectrophotometers have wide applications in metallurgy, pharmacy, food, health, institutes, biological chemistry, life science, petrochemical industry, quality control, environmental protection, etc..

They have adopted the newest microcomputer technology and electronic control system, optimize optical system and structure which can both extend new functions and ensure the accuracy, sensitivity, stability and durability. They have been organised with all kinds of spectrophotometers like UV, visible, single beam, double beam, adjustable slit, xenon and big capacitive touch screen with high resolution and high sensitivity.

Peak Instruments Inc. have steady growth and good reputation in the markets of more than 30 countries, like USA, Canada, Mexico, Argentina, Brazil, Chile, Peru, Ecuador, Spain, Poland, Russia, India, Pakistan, Korea, Thailand, UAE, Turkey, Tunisia, Morocco, Egypt and South Africa.