BioTecNika brings to you Certification Course on “Mass Spectrometry-Based Proteomics Analysis” on special demand by our subscribers.
This Certification program is co-sponsored by PEAK Instruments Inc ,a leading manufacturer of Analytical instruments.
Proteins are essential parts of living organisms, with many functions. The proteome is a term that is used for the entire set of proteins that is or can be expressed by a genome, cell, tissue, or organism at a certain time. The large-scale study of proteins is known as Proteomics. The proteome is not only complex, it is spatially, temporally, and chemically dynamic.
Mass spectrometry is an advanced analytical technique for accurate mass measurement. It does so by producing charged molecular species in vacuum followed by its separation in the magnetic and electric fields on the basis of their mass to charge (m/z) ratio.
Mass spectrometry (MS)-based proteomics is emerging as a broadly effective means for identification, characterization, and quantification of proteins that are integral components of the processes essential for life. Characterization of proteins at the proteome and sub-proteome (e.g., the phosphoproteome, proteoglycome, or degradome/peptidome) levels provides a foundation for understanding fundamental aspects of biology. Among the toolkit of techniques with which proteins can be investigated on a large scale, mass spectrometry (MS) has gained popularity because of its ability to handle the complexities associated with the proteome. Other techniques such as two-dimensional gel electrophoresis (2DE), two-hybrid analysis, and protein microarrays fail to achieve the depth of informative proteome analysis seen with mass spectrometry.
The three primary applications of MS to proteomics are :
- cataloging protein expression
- defining protein interactions
- and identifying sites of protein modification.
The use of MS for proteomics is not the application of a single technique for all purposes but rather a collection of methodologies, each with strengths suited to particular inquiries. MS-based proteomics is increasingly contribute to our understanding of the dynamics, interactions, and roles that proteins and peptides play, advancing our understanding of biology on a systems wide level for a wide range of applications including investigations of microbial communities, bioremediation, and human health.
Fig: General workflow for MS-based high-throughput bottom-up and top-down proteomics.
Common applications and fields that use mass spectrometry:
Proteomics
- Protein structure, function & folding determination
- Identify a protein from the mass of its peptide fragments.
- Detection of specific post-translational modifications in complex biological mixtures.
- Quantitate proteins in a given sample.
- Monitor enzyme reactions, chemical modifications & protein digestion.
Drug Discovery
- Determination & Screening of structures of drugs & metabolites.
Clinical Testing
- In Forensic Analysis
- Detect disease bio-markers (e.g., newborns screened for metabolic diseases).
Genomics
- Sequencing short oligonucleotides.
Environment
- Test food &beverages for contamination, adulteration and
About Peak Instruments Inc.:
PEAK Instruments Inc. is Houston with its head office and warehouse, which is a high tech enterprise integrated with R&D, production, sales and service of spectrophotometers. Spectrophotometers have wide applications in metallurgy, pharmacy, food, health, institutes, biological chemistry, life science, petrochemical industry, quality control, environmental protection, etc..
They have adopted the newest microcomputer technology and electronic control system, optimize optical system and structure which can both extend new functions and ensure the accuracy, sensitivity, stability and durability. They have been organised with all kinds of spectrophotometers like UV, visible, single beam, double beam, adjustable slit, xenon and big capacitive touch screen with high resolution and high sensitivity.
Peak Instruments Inc. have steady growth and good reputation in the markets of more than 30 countries, like USA, Canada, Mexico, Argentina, Brazil, Chile, Peru, Ecuador, Spain, Poland, Russia, India, Pakistan, Korea, Thailand, UAE, Turkey, Tunisia, Morocco, Egypt and South Africa.