This Master certification course on Flow Cytometry will develop practical skills and theoretical knowledge of the modern instrumentation, tools and techniques used to examine cells by flow cytometry. You are encouraged to make links between the various sessions and your research to contextualise knowledge and to assist in unifying the theoretical and practical components and applying them to your research in future .
In this course you will learn about –
- workings of a flow cytometer
- set-up and calibration
- compensation
- fluorescence and fluorochromes
- pre-analytical methods
- quality control
- immunodetection
- data analysis using different softwares
- strategies for the analysis of peripheral blood samples
- applications in immunology.
In addition, you will learn about Multicolor panel design, harmonisation and standardisation of different flow cytometers, advanced data analysis using different softwares, hematopoiesis and analysis of stem cells, immature, and mature cell populations, specific applications in hematology, detailed case studies.
Areas of study will include application and theory of analytical and sorting flow cytometry and the uses of these instruments and techniques in the research laboratory environment. The particular focus of the practical tasks will be tailored to your research field. This may include but need not be limited to immunology, microbiology, pharmacy, plant or zoology studies.
Benefits
After completing this Master certification course of Flow cytometry , you are able to / has knowledge as well as skills related to different aspects .
Knowledge
You gain knowledge in:
- Understanding for basic applications for Flow Cytometry
- which and how many different fluorochromes one can use on a given flow cytometer
- evaluate the quality of flow cytometry data
Skills
You will develop specific skills after completing this particular course:
- Plan and perform basic experiments using Flow Cytometry
- Quality control generated flow cytometry data
- Present flow cytometry data in a scientific way
Competence
- Understanding of basic flow cytometry
- Understanding as to why to use flow cytometry in a certain research question
- Interpret basic flow cytometry data
How Important is the Knowledge Of Flow Cytometry in Research Career?
Flow cytometers were initially used in clinical laboratories to obtain complete cell count. Their reliability and ease of handling increased its demand in research. Now, flow cytometry is an inevitable technique used in biological research for its wide range of applications, especially in the fields of cell biology, immunology, molecular biology, and cancer biology.
Flow cytometric data can be spotted in almost every scientific publication related to cell biology and this demonstrates its worldwide utilization and importance. Moreover, a large proportion of scientific publications related to immunology and biomedical sciences report flow cytometry data. Nowadays, most of the leading universities and biomedical research institutes possess their own flow cytometers, despite its high cost. They are also used for diagnosis and research in hospitals and medical centers worldwide.
The major diagnostic applications of flow cytometer include cell cycle, ploidy, and surface analysis of cancer. They are also used in the study of cell surface markers for leukemias and lymphomas, which could prove useful in their diagnosis. Flow cytometry is the method of choice for measuring CD4 lymphocyte levels in the blood to monitor the progression of AIDS and response to treatment. Less expensive methods are yet not available. In research, clinical applications, and clinical trials, cell sorting turn out to be highly important.
If you are someone aspiring for a research career in biosciences, flow cytometry is one such technology you must be skilled in. Be it an interview for a research position or an actual research project you are handling, you are expected to have full information on flow cytometry, including the working principle and data analysis. To conclude, flow cytometry is indispensable in the field of biology.
Role in Career Advancement
Flow cytometry Master certification course is very essential to all those students or individuals who are planning to have a career in this field in future. So many features are there related to this course that will help you to earn extra points for your resume. The popularity of this analytical tool can be estimated by its range of uses. It has been used for cell counting, cell sorting, measuring cell size and volume, characterizing different cell types in a mixed cell population, analyzing both cell surface and intracellular molecular expression and assessing the purity of isolated subpopulations of cells. Principally checks the fluorescence intensity generated by fluorescent-labeled probes associated with proteins, lipids, DNA or any other specific cellular molecules.
The diversified application of flow cytometry has made it the new-age analytical tool. The popularity of flow cytometry could be well estimated by the growth in the global antibody market and reagent market, which in 2017 amounted to about $945 million and $2.8 billion, respectively. With an increase in demand for primary antibodies, the major application has been observed in the field of flow cytometry.
First of all many job openings are available in research in labs, academic sector and commercial sector as research fellow- JRF/SRF, research associate, research scientist, Post-Doctoral Fellow (PDF) and lab technician in universities, research institutes, and hospitals . Many pharmaceutical and clinical companies also employ persons with expertise in flow cytometry specifically those associated with antibody maker development, designing disease targets and drug targets such as cancer, involved in the diagnosis, clinical trials and quality control and assurance. Moreover, other job opportunities are also available apart from direct research or machine operation.
Some of the companies frequently hiring experts in flow cytometry are
- Boehringer Ingelheim India Pvt Ltd
- Thermo Fisher Scientific
- Syngene International Ltd
- AstraZeneca Pharma India Ltd
- Iqvia
- Pfizer Inc
- Nestle Ltd.
- Apex Services
- BD India Pvt Ltd
- MilliporeSigma, Merck Ltd
- Phenomenex Inc.
- Cancer Genetics Inc.
- Averinbiotech Pvt Ltd
- Becton Dickinson India Pvt Ltd.
- Thyrocare Technologies Ltd and many more.